Parenchyma-sparing anatomical resection is recommended in patients with hepatocellular carcinoma due to the presence of underlying liver disease. More precise hepatectomy has been enabled by recent technical advancements, including negative staining with indocyanine green following ligation of the corresponding Glissonean pedicle, which offers intraoperative guidance by delineating the resection plane in real-time. Herein, we present a case of laparoscopic left lateral sectionectomy that used this staining technique.